Drug Abuse Test Kit BAR (Barbiturates) Rapid Diagnostic Test strip
, urine drug test, cut-off 300ng/ml
BAR Rapid Diagnostic Test/ Barbiturates Rapid Diagnostic Test
Barbiturates are central nervous system depressants. They are used
therapeutically as sedatives, hypnotics, and anticonvulsants.
Barbiturates are almost always taken orally as capsules or tablets.
The effects resemble those of intoxication with alcohol. Chronic
use of Barbiturates leads to tolerance and physical dependence.
Short acting Barbiturates taken at 400 mg/day for 2-3 months
produce a clinically significant degree of physical dependence.
Withdrawal symptoms experienced during periods of drug abstinence
can be severe enough to cause death. Only a small amount (less than
5%) of most Barbiturates are excreted unaltered in the urine. The
detection period for the Barbiturates in the urine is 4-7 days.1
The BAR One Step Barbiturates Test Strip (Urine) is a rapid urine
screening test that can be performed without the use of an
instrument. The test utilizes a monoclonal antibody to selectively
detect elevated levels of Barbituratess in urine. The BAR One Step
Barbiturates Test Strip (Urine) yields a positive result when
Barbituratess in urine exceed 300 ng/mL
The BAR One Step Barbiturates Test Strip (Urine) is a rapid
chromatographic immunoassay based on the principle of competitive
binding. Drugs which may be present in the urine specimen compete
against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary
action. Barbiturates, if present in the urine specimen below 300
ng/mL, will not saturate the binding sites of antibody-coated
particles in the test strip. The antibody-coated particles will
then be captured by immobilized Barbiturates conjugate and a
visible colored line will show up in the test line region. The
colored line will not form in the test line region if the
Barbiturates level exceeds 300 ng/mL because it will saturate all
the binding sites of anti-Barbiturates antibodies.
A drug-positive urine specimen will not generate a colored line in
the test line region, while a drug-negative urine specimen or a
specimen containing a drug in a concentration less than the cut-off
will generate a colored line in the test line region. To serve as a
procedural control, a colored line will always appear at the
control line region, indicating that proper volume of specimen has
been added and membrane wicking has occurred.
DIRECTIONS FOR USE
Bring tests, specimens, and/or controls to room temperature
(15-30°C) before use.
Remove the test from its sealed pouch, and place it on a clean,
level surface. Label the test with patient or control
identification. For best results, the assay should be performed
within one hour.
Using the provided disposable pipette, transfer 3 drops of specimen
(approximately 120 µL) to the specimen well (S) of the device and
start the timer.
Avoid trapping air bubbles in the specimen well (S), and do not add
any solution to the result area.
As the test begins to work, color will migrate across the membrane.
Wait for the colored band(s) to appear. The result should be read
at 5 minutes. Do not interpret the result after 8 minutes
INTERPRETATION OF RESULTS
NEGATIVE:* A colored line appears in the Control region (C) and colored lines
appears in the Test region (T). This negative result means that the
concentrations in the urine sample are below the designated cut-off
levels for a particular drug tested.
*NOTE: The shade of the colored lines(s) in the Test region (T) may
vary. The result should be considered negative whenever there is
even a faint line.
POSITIVE: A colored line appears in the Control region (C) and NO line
appears in the Test region (T). The positive result means that the
drug concentration in the urine sample is greater than the
designated cut-off for a specific drug.
INVALID: No line appears in the Control region (C). Insufficient specimen
volume or incorrect procedural techniques are the most likely
reasons for Control line failure. Read the directions again and
repeat the test with a new test card. If the result is still
invalid, contact your manufacturer.
A procedural control is included in the test. A line appearing in
the control region
(C) is considered an internal procedural control. It confirms
sufficient specimen volume, adequate membrane wicking and correct
Control standards are not supplied with this kit. However, it is
recommended that positive and negative controls be tested as good
laboratory practice to confirm the test procedure and to verify
proper test performance.
A drug-free urine pool was spiked with Barbiturates at the
following concentrations: 0 ng/mL, 150 ng/mL, 225 ng/mL, 300 ng/mL,
375 ng/mL, 450 ng/mL and 600ng/mL. The result demonstrates > 99%
accuracy at 50% above and 50% below the cut-off concentration.
Results are presented in Table 2 below.
Table 2: Analytical Sensitivity Summary
|BAR Concentration (ng/mL)||Percent of Cut-off||n||Visual Result|
Conclusion: As indicated in table above: all specimens with BAR concentration
equal to or lower than 150ng/mL show negative results, all
specimens with BAR concentration of 300ng/mL are identified as
“+/-”, and all specimens with BAR concentration equal to or higher
than 450ng/mL showed positive results. Therefore, the cut-off
concentration of the BAR One Step Test Strip (Urine) is determined
to be 300ng/mL BAR.
Table 3 lists the compounds that are positively detected in urine
by the BAR One Step Barbiturates Test Strip (Urine) at 5 minutes
and the concentrations at which they are detected.
Table 3: Analytical Specificity
|Compound||Concentration (ng/mL)||Compound||Concentration (ng/mL)|
|ORIENT NEW LIFE MEDICAL CO., LTD.|
|Email:||Jerry @ newlifebiotest .com|